Part:BBa_K1026009:Design

A rationally designed promoter negative regulated by CcaR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 42
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We discovered a segment around the -35 region of BBa_J23107 that is almost identical to G-box, the binding region of CcaR on PcpcG2. So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter. Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization.

Source

Synechocystissp. PCC 6803